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You will notice some difference in speed. Speed is not only dependent on the RPM, but the cache as well. So it would make an even bigger difference if the 5400 RPM drive had a 2 MB cache and the 7200 RPM drive had an 8 MB cache. Both will work fine if they are the correct interface.
Note that when I say "speed", I am talking about real life applications, not benchmarking.
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