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Remember that the memory controller is built into the AMD64 so it can depend on the generation of processor used.
On my A8V Deluxe, you don't loose dual channel with 4 sticks of RAM.
I'm betting the same for the A8V-E.
However, you might loose the 400FSB to your memory.
It'll default to 333Mhz with 4 sticks of double sided RAM.
You may have to manually adjust the speed. Use memtest86 after you do just to make sure things are running ok.
My 1st gen 3500+ tends to not like running at 400FSB with 4 sticks, but my 3800+ X2 has no problem with it. This is with the same A8V Deluxe board with the same memory.
Good Luck.
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ASUS Board: P5WD2-E Premium - BIOS 404 CPU: Pentium-D 840 (3.2Ghz) Heatsink: Thermaltake Tower 112 Memory: Kingston KVR667D2N5K2 - 2Gig DDR2@667 Kit Video: Matrox APVe Boot Drive: WDC Raptor 36Gig on ICH7 SATA1 RAID 0: x2 Hitachi T7K250 250Gig on ICH7 SATA 3&4 PSU: Enermax Liberty 620W
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